New blog post: Driving metabolic pathways on with sunlight – University of Copenhagen

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04 December 2015

New blog post: Driving metabolic pathways on with sunlight

New blog post about using photosynthetic electrons to drive cytochrome P450 enzymes in cyanobacteria based on article by Copenhagen Plant Science Centre researchers. The blog was originally posted on the PLOS Synbio blog. You can read the highlights here:

Most life forms are directly or indirectly dependent on photosynthesis. The question is, can we exploit sunlight more broadly than in carbohydrate production, making it effectively a synthetic biology part? As an answer to this,  researchers from PHOTO.COMM, Plant Power and Copenhagen Plant Science Centre published an article on using photosynthetic electrons to drive cytochrome P450 enzymes in cyanobacteria.

© 2014 Lassen et al. Anchoring a Plant Cytochrome P450 via PsaM to the Thylakoids in Synechococcus sp. PCC 7002: Evidence for Light-Driven Biosynthesis. PLoS ONE 9(7): e102184. Under Creative Commons Attribution License.

Cytochrome P450s are enzymes that oxygenate organic compounds stereo-specifically and are involved in numerous metabolic routes. Their reaction mechanism requires electrons, usually obtained from redox cofactors such as NADPH.

This availability of reducing power is often the bottleneck in heterologous expression plant pathways in microbes and the biosynthetic steps catalyzed by P450s required laborious optimization. There might be however another way to overcome this hurdle, the answer could lie in photosynthesis.

The subject of the latest research article the CPSC vice-head, Poul Erik Jensen’s group is around harnessing sunlight and redirecting it towards desired metabolic compounds. The paper, titled “Metabolic engineering of light-driven cytochrome P450 dependent pathways into Synechocystis sp. PCC 6803”, was recently published in Metabolic Engineering. In this work, the researchers engineer Synechocystis sp. PCC6803, a popular cyanobacterium model, to produce dhurrin (a cyanogenic glucoside from Sorghum bicolor).

“Cyanobacterium-inline” by Kelvinsong – Own work. Licensed under CC BY-SA 3.0 via Wikimedia Commons.

Sorghum utilizes dhurrin as a defense compound: when the leaf tissues are mechanically disrupted (chewed), dhurrin degrades and hydrogen cyanide releases, poisoning the unfortunate herbivores. Its biosynthesis commences from tyrosine, and involves two distinct cytochrome P450s and a glycosyltransferase. Although dhurrin has no commercial use, its biosynthesis has evolved into a model pathway for understanding plant P450 functionality (I refer the curious reader to the work of Professor Birger Møller‘s research and the University of Copenhagen Center for Synthetic Biology).

More interestingly, the dhurrin P450s were used to demonstrate that it is possible for P450s to obtain electrons directly from the photosynthetic apparatus. Eukaryotic P450s are normally located in the endoplasmic reticulum and rely on a NADPH-dependent dedicated oxidoreductase as a redox partner. However, in vitro and in vivo experiments have shown that if the P450s localize in the proximity of the photosystem I, they can retain activity by gaining electrons from photoreduced ferredoxin, thus bypassing the specialized reductase requirement. Since this concept worked quite well in plant chloroplasts, the researchers saw no reason that this principle is not transferable to cyanobacteria.

Schematic representation of the dhurrin pathway, localized in the thylacoid membranes, using ferredoxin as electron donor. Figure reproduced from Nielsen et al, ACS Synth Biol. 2013 Jun 21;2(6):308–15. under ACS AuthorChoice/Editors’ Choice Usage Agreement.

Coming back to the research paper, the authors introduced the three enzyme sequences of the dhurrin pathway into a self-replicating vector as an operon, their expression controlled by a strong inducible promoter.

Under theophyline induction, cyanobacteria produce dhurrin and excrete most of it to the growth medium. The productivity was also tested in 8 Liter bioreactors, where dhurrin accumulation reached 3.2 mg dhurrin L-1OD-1 after 7 days of cultivation.

The effect of dhurrin production on cell fitness was also tested. When the whole pathway was expressed, there was a small delay in cell growth. But the strains expressing only the two first enzymes (the p450s without the glycosyltransferase) displayed a severe poisoning phenotype. Electron microscopy revealed rearrangements of the thylacoid structures and the lack of glycogen granules. These side-effects were not observed in the full pathway-expressing strain, suggesting that some intermediate may have toxic effect and that the glycosilation is crucial for the detoxification and secretion of this potential poison.

Hijacking electrons from photosynthesis is a promising bioengineering alternative, especially in the cases where reducing power and co-factor availability are limiting. This study shows that Synechocystis is receptive to this practice, and paves the way for further metabolic engineering work, aiming to produce more and commercially interesting compounds.

Cyanobacteria are prominent vessels for synthetic biology approaches, recently receiving attention from NASA as polymer construction hosts. Even though it might be some time before seeing photosynthetic organisms doing large scale production in space (or in Mars, according to our 2015 iGEM SpaceMoss team), the principle remains the same: light, CO2 and water—feedstock plentifully available in a resource-limited planet—are captured by photosynthetic microbial cell factories to produce any fuel, nutrient, or pharmaceutical.

Read more about Professor Jensen’s research here.

Disclaimer: This post originally appeared in the PLOS Synbio blog, find the original version here. We kindly thank the community editors for reprint permission. 

Written by Konstantinos Vavitsas, PhD student at the Copenhagen Plant Science Centre, University of Copenhagen, working on the photosynthetic production of high-value compounds (Plant Power project). Find him on LinkedIn or follow him on Twitter.