Talk by Piotr Gawroński – University of Copenhagen

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Copenhagen Plant Science Centre > Event calendar > 2015 > Talk by Piotr Gawroński

Talk by Piotr Gawroński

ISOCHORISMATE SYNTHASE 1 is required for thylakoid organization, optimal plastoquinone redox status and state transitions in Arabidopsis thaliana

Piotr Gawroński is working in Prof. dr eng. Stanisław Mariusz Karpiński’s lab at The Laboratory of Physiomics and Crop Design at Warsaw University of Life Sciences in Poland. On 24 March he will give a talk at Copenhagen Plant Science Centre.

Abstract: ISOCHORISMATE SYNTHASE 1 (ICS1) is a crucial enzyme in the salicylic acid (SA) synthesis pathway, thus it is important for immune defences. The ics1 mutant is used in experiments concerning plant-pathogen interactions and ICS1 is required for appropriate hypersensitive disease defence response. However, ISC1 also takes part in the synthesis of phylloquinone, which is incorporated into photosystem I and is an important component of the photosynthetic electron transport in plants. Therefore we performed photosynthetic and molecular analysis of the ics1 mutant in comparison to SA-degrading transgenic NahG and wild type Arabidopsis thaliana plants. 

Photosynthetic parameters in the ics1 mutant, when compared to the wild-type, were changed in a manner observed previously for state transition-impaired plants (STN7 kinase recessive mutant, stn7). In contrast to stn7, deregulation of the redox status of the plastoquinone pool (measured as 1–qp) in ics1 showed significant variation depending on the leaf age. SA-degrading transgenic NahG plants targeted to the cytoplasm or chloroplasts displayed normal (wild type-like) state transition.

However, ics1 plants treated with the phylloquinone precursor displayed symptoms of phenotype reversion towards wild type. ics1 also showed altered thylakoid structure with increased number of stacked thylakoids per granum what supports the role of ICS1 in regulation of state transition. The presented results suggest the role of ICS1 in integration of the chloroplast ultrastructure, the redox status of the plastoquinone pool and the photosystems organisation that are important for optimal immune defence and light acclimatory responses.

Piotr Gawroński is working as a postdoc in Professor Stanisław Mariusz Karpiński’s lab at The Laboratory of Physiomics and Crop Design at Warsaw University of Life Sciences in Poland. Piotr will be staying at CPSC as a guest scientist in a collaboration between Professor Karpiński’s and Profofessor Dario Leister’s labs.