Talk by Peter Kindgren about PPR proteins – University of Copenhagen

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Copenhagen Plant Science Centre > Event calendar > 2015 > Talk by Peter Kindgren...

Talk by Peter Kindgren about PPR proteins

On 16 February 2015 at 11.00-12.00 Peter Kindgren from University of Western Australia, ARC CoE Plant Energy Biology will give a talk at Thorvaldsensvej 40, 1871 Frederiksberg C, room H117-3. The topic of the talk is: PPR proteins: an emerging tool to control user-defined RNA targets.

Abstract: Pentatricopeptide repeat (PPR) proteins bind RNA and determine a wide range of RNA processing events required to prepare plant organellar RNA for translation, such as stability, splicing and editing. PPR motifs are capable of sequence-specific interaction with the target RNA via interactions involving 2-3 amino acids in each motif that recognize individual nucleotides in the RNA target.

By systematically mutating the RNA-interacting amino acids in CLB19, which edits rpoA and clpP in plastids, and the nucleotides in its target RNAs, we have confirmed the exact binding sites and the residues that determine target specificity. We have shown that it is possible to predictably alter the binding preferences of RNA editing factors in vitro and in vivo. Coupled with this, our studies explain what the functions are for the additional C-terminal domains in RNA editing factors.

Both the E- and the DYW-domain are required for editing and the DYW-domain is highly likely to be deaminase domain responsible for the C-to-U conversion while the E-domain is required for efficient binding to the RNA target. PPR proteins have the potential to be a future tool for manipulating RNA species in organelles. To achieve this, we have started working on synthetic PPR proteins built up by a tandem array of PPR-motif derived from a consensus sequence. The use of manipulated natural and synthetic PPR proteins for controlling user-defined RNA targets will be further discussed.